Cordyceps Blog

On Mice and Moods

Submitted by cordyceps on
I just recently came across this research published already in 2007 on Cordyceps sinensistested as an antidepressant on mice. Past experiments using anti-depressants have shown that mice suspend their tails up in the air when they are in a better mood. So tails up apparently can be used to access the potential of a drug as antidepressant. I pasted the summary in and set a link to the complete paper.

 

Antidepressant-Like Effect of Cordyceps sinensis in the Mouse Tail Suspension Test
Koji NISHIZAWA, Kosuke TORII, Aya KAWASAKI, Masanori KATADA, Minoru ITO,Kenzo TERASHITA,Sadakazu AISO and Masaaki MATSUOKA
Biol. Pharm. Bull. 30(9) 1758—1762 (2007)
 
link
Summary
Cordyceps sinensis (CS) has been known as a component of traditional medicines that elicit various biological effects such as anti-fatigue, immunomodulatory, and hypoglycemic actions. Since it has been well-established that fatigue is closely related to depression, we used the tail suspension test (TST) in mice to examine the antidepressant-like effects of hot water extract (HWCS) and supercritical fluid extract (SCCS) of CS. Immobility time in the TST was reduced by administration of SCCS (2.5—10 ml/kg, p.o.) dose-dependently though it was not reduced by treatment with HWCS (500—2000 mg/kg, p.o.). Neither HWCS nor SCCS altered locomotor activity in the open field test, excluding the possibility that the effect of SCCS is due to activation of locomotion. Pretreatment with prazosin (an adrenoreceptor antagonist) or sulpiride (a dopamine D2 receptor antagonist) reduced the effect of SCCS on the immobility time. In contrast, pretreatment with p-chlorophenylalanine (p-CPA, a serotonin synthesis inhibitor) did not alter the anti-immobility effect of SCCS. The last finding is consistent with an additional observation that SCCS had no effect on head twitch response induced by 5-hydroxy-L-tryptophan in mice. Taken altogether, these results suggest that SCCS may elicit an antidepressant-like effect by affecting the adrenergic and dopaminergic systems, but not by affecting the serotonergic system.

Key words Cordyceps sinensis; depression; tail suspension test; noradrenaline; dopamine

Here a picture from a mouse (or pika) that observed me with big eyes while I was first  photographing and then digging out a Cordyceps at Kongpo Barla. Interestingly the fruiting body already had some gnawing traces. I hope the poor mouse managed to keep its tail up after seeing me stealing its yartsa gunbu.

 

 



Cordyceps interview with National Geographic Weekend

Submitted by cordyceps on

When I was in Washington DC in late March to present on Cordyceps sustainability at the World Wildlife Fund headquarters "Cordyceps - Tibet's Golden Fungus: Bane or Blessing? I was also interviewed by Boyd Matson from National Geographic Weekend, a radio show. 

The subject was, surprise! surprise! Caterpillar fungus. We talked about its role in Tibet, sustainability issues and its perceived and actual "potency". NG weekend was so kind to send me an edited ten minute audio file you can listen to here. I thought it is quite entertaining and informative, but I might be biased......

Daniel during an interview in Shunlung in April 2002

Mushroom Cooking in Tibet Video

I produced my first video for Youtube. It is on Kar Sha cooking. It shows how Meadow mushrooms (Agaricus campestris) are cooked the traditional way with tsampa, butter and salt. The caps are put gills up on the fire in Tibet. It also includes some nice landscape scenes from Kham as well.  It is still a little rough, but at only 3 minutes not too torturous   ;0}

I have much more video footage from my MushRoaming travels in Tibet [and Ecuador], but I am still very slow in editing, I really need a crash course to get all the great stuff out. 




Shimpu du!  Yummie!

New Article on Mushrooms in Bhutan

My article on Mushrooms in Bhutan entitled: Bhutan's Buddha Mushroom was finally published in Fungi (No.4.1). It describes then local mushroom markets, especially the Matsutake market and collection, deals with "Yartsa goenbub" - Cordyceps sinensis in Bhutan, and a range of other edibles favored by the Bhutanese.

 

Caterpillar Fungi from Ecuador's Rain Forest

Submitted by cordyceps on
Got back this week from the MushRoaming Ecuador Tour I organized with Larry Evans. We all had great fun and learned a lot about the funga in the Amazon and many other interesting subjects related to ecology and culture. We are offering another tour in early 2013 to Bolivia

Here a few new Cordyceps / Ophiocordyceps photos from specimen I found in the tropical forests of Ecuador, what an incredible place, what enormous biodiversity!


 

We found several Ophiocordyceps with bright red perithecia, looking quite similar and being closely related to Ophiocordyceps australis [formerly: Cordyceps australis]. 

Below an Ophiocordyceps on a weevil with a whole bunch of fruiting bodies growing out of it that is quite similar to Ophiocordyceps curculionum. However DNA analysis by Joey Spatafora, OSU Corvallis have shown that this is most likely a new species closely related to O. curulionium.


Close ups of the perithecia of Ophiocordyceps australis. What a great red these diminutive stromata display. The perithecia look close to maturity.



Below the fungus looks like a proper Ophiocordyceps australis, the most common pathogen in the neotropics. It infected a Conga ant (Paraponera sp.), which is one of the biggest ants in the Amazon. Fungus and ant measure about 12 to 15 cm (5 to 6 in). George Yager spotted the single stroma first, I thought it was a tiny red Mycena and was ready to move on, but Larry Evans recognized the Cordyceps. 



This ant was tiny, it measured only 1.2 cm (0.5 in) and you can imagine how small the fruiting body is. I only found it when I was taking multiple pictures of another O. australis and saw a tiny red spot down below.
I am not sure if this is a specimen of  Ophiocordyceps australis itself, the red fertile tissue of the fruiting body is less rounded than the typical O. australis. 

Note the white growths on the hind leg. They look like conidiomata, which produce spore-like conidia. Conidia are asexual spores, basically reproductive cell clones. Often Cordyceps are only reproducing through conidia. A fungal organism that only produces conidia is known as an anamorph. Anamorphs do not grow stromata or fruiting bodies, only teleomorphs do. Usually lack of fruiting bodies makes identification of a Cordyceps much more difficult.



Near the Umbuni waterfalls I found this creature. At first there were just a few brightly orange stromata visible coming out of a decaying trunk, a favorite site for Cordyceps to direct its prey to dig in before kill off. 


Once the fungus cum coleopteran [=beetle] larva was excavated this wild gestalt became visible:

According to Prof. Spatafora this fungus is a member of Metacordyceps. However, these species seem to acquire hyperparasites quite easily, making the collection of molecular data difficult. A possible name for this morphology is Metacordyceps martialis.


Another Cordyceps, according to Joey Spatafora, OSU mycologist and Cordyceps expert, possibly a Metacordyceps, "Joey wrote: "There are a number of Asian species with this overall morphology (e.g. M. liangshanesis) but I do not know of anything from South America."  Here a recent article on the taxonomic status of Metacordyceps, one of the authors being Joey Spatafora.
White fertile stroma tissue is not too common. It is growing on a lepidopteran larva that was also buried in a decaying trunk. Check out the white perithecia.

2020 update: This species is now described as Nigelia martiale 


Close up of the stroma

Cordyceps from Mindo, western slope of the Andes

Below a Cordyceps anamorph that could be Isaria tenuipes, a.k.a. Paecilomyces tenuipes, but there are also other morphologically similar anamorphs. We found it in on trunk near Mindo.


 
An Ophiocordyceps/  Cordyceps I found in Mindo, sitting on top of an decaying, moss covered trunk. The site was in the cloud forest on the western slope of the Andes in Ecuador. I will try to find out if has been described already.
 
Close ups of the perithecia of this Ophiocordyceps.

And finally an unidentified Cordyceps relative we found on the wall in our hobbit-style guest house in Mindo. Just wouldn't find a Cordyceps on your wall if it was an upper class hotel, right?



First uploaded 3-6-2011, last update 11-30-2011

New Drug derived from (Ophio-) Cordyceps sinensis

Submitted by cordyceps on

It took me four months to finally add a new entry here yesterday - I spend much more time on my web pages at www.MushRoaming.com - and today this comes through the news ticker: 

Himalayan Fungus Aids Mitsubishi Tanabe Sales With Multiple Sclerosis Drug 
written by Kanoko Matsuyama in Tokyo for Bloombergs.


Apparently, Gilenya, as it is marketed by Novartis, a new promising Multiple Sclerosis drug has been developed from Cordyceps sinensis. Twenty five years ago the researcher Tetsuro Fujita had the idea to use Cordyceps since it must suppress the immune system of the ghost moths it feeds on in the Highlands of Tibet and the Himalayas. 

We do not know if any real Cordyceps is used as raw material, but the price of a monthly dose of $3000 could suggest that, however pharmaceutical companies probably would charge the same amount if the base was regular straw and the patients in dire need. The analysts are hearing  the cash registers ringing out loud . It is speculated that they might make soon 5 billion a year in global annual sales and make it under the top ten drugs, no not most expensive, just highest grossing drugs. 

The article mentions the meaning of the Chinese and Japanese name of Cordyceps as "Winter worm, summer plant", in Chinese it is cao = grass, but fails to mention that these names are translations of the original Tibetan name Yartsa gunbu "summer grass, winter worm". It would have been nice if Tibetan medicine gets the credit it deserves, since it was first used in Tibet. And also its first record dates to the late 15th century doctor Zurkhar Nyamnyi Dorje. And what about not only a credit to the roots of this medicinal, but to Tibetan Medicine for having discovered the medicinal value of this weird organism? And once in the dream state, what about a slice of Mitsubishi Tanabe & Novartis pharma profit pie for the further development of Tibetan medicine?   

Image of Cordyceps subsessilis from D. Shimizu and K. Kobayashi 1997. Illustrated Vegetable Wasps and Plant Worms in Colour, Tokyo.


Interestingly, no mentioning in the article of Ciclosporin, another famous drug that has been developed from Cordyceps, but another species Cordyceps subsessilis. Ciclosporin, is used to suppress immune reaction after organ transplants, a procedure not possible without immuno-suppression. It was originally derived from Tolypocladium inflatum and only later recognized as the true anamorph [meaning an asexually often mold like state] of C. subsessilis by Cornell mycologist Kathy Hodge and others.

Zurkhar Nyamnyi Dorje in a contemporary Yartsa Gunbu Thangka



The Men-tsee-khang webpage reports about Zurkhar Nyamnyi Dorje

Zurkhar Nyamnyi Dorjee was born to Rigzin Phuntsok and the daughter of Kunkyen Tashi Namgyal in the Earth Sheep year. He learned Buddhist philosophy and medicine from many renowned scholars and, at the age of 16, he wrote Manngag-Jewa-Ringsel (Pith Instructions, Relics in Crores, [better translated as "Instructions on a Myriad of Medicines"]) and many other treatises. He was the founder of the Zurlug tradition of Tibetan medicine. Later, his grandson Zurkhar Lodoe Gyalpo wrote a commentary on rGyud-bzhi called Mepoi Zallung (Oral Instructions of my Forefathers).

 

Cordyceps Mishaps in the Mean Stream News

Submitted by cordyceps on

Caterpillar fungus made it in the main stream news in January. From the BBC to New York Post . The articles focused on a sad story of a mass murder in Nepal's Annapurna region. In a nutshell the sad story went like this: A group of 7 non-local poachers were found sneaking into the village's Cordyceps habitat and was confronted by 65 local men. One of the poaching Gorkhas got killed, so some came up with the idea to kill them all and pretend nothing ever happened. Well, that did not work. Now 35 of them are accused of murder. The story was originally brought to light to western readersby Jamie James article "Nepal's Aphrodisiac War" published in Men's Journal in May 2010, it previously was reported in South Asian media.

Not surprising, but still disappointing, the media attention focused on the dark side of the Yartsa gunbu story, or Yarsagumba as it is known in Nepali. The BBC piece by Joanna Jolly was entitled: "Yarsagumba - Curse of Himalayan Annapurna Region". Sadly, each year some people get killed when emotions run high in the context of harvesting this extremely precious fungus. We are talking of an annual harvest worth in the hundreds of millions, and these are not Rupees or Yuan, but Dollars or Euros. Real big money in this otherwise impoverished region of Tibet and the Himalayas. Taking these astronomical values into account in an industry that takes place in extremely remote areas with very little oversight, it is surprising how few fatal incidents happen each year.  In regard of value generated and transferred, the Yartsa gunbu fever is probably best compared to an annually reoccurring gold rush. And gold rushes are an economic phenomenon usually accompanied by many, many more fatalities. 

In general, I am very impressed how non-combative the whole collection is and how peaceful the collection season passes each year. And, yes, each fatality is very sad and unnecessary. Already some stories have been published, for example from Dzato County in South Qinghai where some local officials got killed in 2005 over selling harvesting permits to outsiders without consulting the locals or sharing profits with locals. I also mention a few other cases in my 2008 Economic Botany article on the Yartsa gunbu economy in Tibet.

Another sad event I heard about took place in Nagchu in 2009. A local herder and a collector got in a fight. The herder insisted it was not acceptable to pick Yartsa gunbu around the shores of a sacred lake. The devout person saw the purity of the site being spoiled. A fight ensued and one of them got killed, thus in the end human blood spoiled the water of the sacred lake. 

This raises the question why do many Tibetan people, be they under Chinese or Nepali rule, regard the collection of Yartsa gunbu as a source of bad karma? This perspective stems from a traditional Tibetan taboo, described in Namkhai Norbu's book "journey among Tibetan nomads" (2002) that digging up the ground for roots or mineral upsets the local spirits, which then will strike the transgressor, his or her clan and their livestock with disease and other misfortune. Furthermore, Yartsa gunbu is perceived as a living creature, the larva is still alive from a traditional perspective, thus harvesting caterpillar fungus is an act of killing. However from a scientific view the killing of the insect was already done by the fungus, the collectors are now taking the life of the insect killing fungus, an act that usually receives much less attention, since mushrooms are perceived as a less conscious life-form than animals.

Back to recent articles on Yartsa gunbu; Much relevant information on Caterpillar fungus is contained in an English language article by French Observer 24 entitled:  , worth its weight in gold".


Here an image of maybe Tibet's first Yartsa Gunbu Thangka, I hope I did not upset any traditionalists commissioning this scroll painting including the Medicine Buddha - Sanggye Menla སངས་རྒྱས་སྨན་བླ། and Zurkhar Nyamnyi Dorje, a famous Tibetan Physician, who wrote about Yartsa Gunbu in the 15th Century, the first known record of Ophiocordyceps sinensis.