cordyceps's blog

Cordyceps cicadae: liver cancer fighting propensities

Interestingly here an open access paper that looks into the effects on liver cancer cells of one of the "minor" medicinal Cordyceps species, Cordyceps cicadae. There has been a bunch of research into C. cicadae, especially regarding kidney support and also as an TCM asthma remedy (see refernces of this paper). Anyways, some years ago I have bought a few specimen for very cheap in a locally sourced materia medica & souvenir store in Gyalthang, currently Shangrila, Deqen Tibetan Autonomous Prefecture Yunnan. And the low price is one of the arguments for this Cordyceps, since Tibetan Yartsa gunbu (Ophiocordyceps sinensis) is just astronomically priced.

 

Cordyceps cicadae induces G2/M cell cycle arrest in MHCC97H human hepatocellular carcinoma cells: a proteomic study

Hualin Wang12Jing Zhang1Wai-Hung Sit1Chung-Yung Jetty Lee1 and Jennifer Man-Fan Wan1*

Abstract

Background

Cordyceps cicadae is a medicinal fungus that is often used for treating cancer. However, the anticancer mechanisms of C. cicadae are largely unknown. This study aims to investigate the anticancer mechanisms of C. cicadae against hepatocellular carcinoma cells in vitro using a proteomic approach.

Methods

Human hepatocellular carcinoma MHCC97H cells were treated with a water extract of C. cicadae(0, 100, 250, 500, and 1000 μg/mL) for 48 h and harvested for cell viability assays. The significant differences in protein expression between control and C. cicadae-treated cells were analyzed by two-dimensional gel-based proteomics coupled with matrix-assisted laser desorption ionization-time of flight mass spectrometry. Flow cytometry analysis was employed to investigate the cell cycle and cell death. The anticancer molecular mechanism was analyzed by whole proteome mapping.

Results

The water extract of C. cicadae (0, 100, 250, 500, and 1000 μg/mL) inhibited the growth of MHCC97H cells in a dose-dependent manner via G2/M phase cell cycle arrest with no evidence of apoptosis. Among the identified proteins with upregulated expression were dynactin subunit 2, N-myc downstream-regulated gene 1, heat shock protein beta-1, alpha-enolase isoform 1, phosphatidylinositol transfer protein, and WD repeat-containing protein 1. Meanwhile, the proteins with downregulated expression were 14-3-3 gamma, BUB3, microtubule-associated protein RP/EB family member 1, thioredoxin-like protein, chloride intracellular channel protein 1, ectonucleoside triphosphate diphosphohydrolase 5, xaa-Pro dipeptidase, enoyl-CoA delta isomerase 1, protein-disulfide isomerase-related chaperone Erp29, hnRNP 2H9B, peroxiredoxin 1, WD-40 repeat protein, and serine/threonine kinase receptor-associated protein.

Conclusion

The water extract of C. cicadae reduced the growth of human hepatocellular carcinoma MHCC97H cells via G2/M cell cycle arrest.

Cordyceps cicadae

Last edited Thu, 05/29/2014 - 10:37

Cordyceps acridophila - A New Grasshopper Cordyceps

With the help of Tatiana Sanjuan, Colombian Cordyceps expert and friend, my photo of Cordyceps acridophila growing on a grasshopper (Agriacris plagiata, Orthoptera: Romaleidae) made the cover of Mycologia for an article on new Amazon Cordyceps species, what an honor! The article is entitled: 

Entomopathogens of Amazonian stick insects and locusts are members of the Beauveria species complex (Cordyceps sensu stricto)
by Tatiana Sanjuan, Javier Tabima, Silvia Restrepo, Thomas Læssøe, Joseph W. Spatafora, and Ana Esperanza Franco-Molano
In: Mycologia 2014 106:260-275

When Nicole Cook found this Cordyceps during our January 2012 Mushroaming Bolivia trip, it was still known as Cordyceps locustiphila

Cordyceps acridophila on grasshopper

 

Abstract: In the Amazon the only described species of Cordyceps sensu stricto (Hypocreales, Cordycipitaceae) that parasitize insects of Orthopterida (orders Orthoptera and Phasmida) are Cordyceps locustiphila and C. uleana. However, the type specimens for both taxa have been lost and the concepts of these species are uncertain. To achieve a more comprehensive understanding of the systematics of these species, collections of Cordyceps from the Amazon regions of Colombia, Ecuador and Guyana were subjected to morphological, ecological and molecular phylogenetic studies. Phylogenetic analyses were conducted on partial sequences of SSU, LSU, TEF, RPB1 and RPB2 nuclear loci. Two new species are proposed including C. diapheromeriphila, a parasite of Phasmida, and C. acridophila, a parasite of the superfamily Acridomorpha (Orthoptera), which is broadly distributed across the Amazon. For C. locustiphila a lectotypification and an epitypification are made. Cordyceps locustiphila is host specific with Colpolopha (Acridomorpha: Romaleidae), and its distribution coincides with that of its host. The phylogenetic placement of these three species was resolved with strong support in the Beauveria clade of Cordyceps s. str. (Cordycipitaceae). This relationship and the morphological similarity of their yellow stromata with known teleomorphs of the clade, suggest that the holomorphs of these species may include Beauveria or Beauveria-like anamorphs. The varying host specificity of the beauverioid Cordyceps species suggest the potential importance of identifying the natural host taxon before future consideration of strains for use in biological control of pest locusts. 

Key words: Anamorph-teleomorph connection, entomopathogenic fungi, host specificity, Neotropics, Orthoptera, phylogeny

 

Here another shot of the same unlucky grasshopper seen in Madidi National Park, Beni, Bolivia

Last edited Sat, 05/21/2016 - 00:16

Yartsa gunbu harvest 2013

 

 

Apparently the Agriculture Department of Tibet Autonomous Region has published the 2013 Yartsa gunbu harvest quantities [See the press article from CCTV news webpage].
In brief 2013 a harvest of 53.7 tons was recorded.
Since 1999 the annual Yartsa gunbu harvest is recorded in Tibet AR, a very important contribution to understanding the Cordyceps phenomenon, but unfortunately an unique achievement. Tibetan areas in Sichuan, Qinghai, Yunnan and Gansu do not record their annual harvest, which is especially disappointing in the case of Qinghai Province, whose production often is higher than Tibet AR.

To put the 53.7 tons harvested in 2013 into historic context, since 1999 the annual harvest fluctuated between 33 t to 57 t, on average it was 44 t (see graphic below, blue figures on left). So clearly 2013 was a productive year. And it is great to hear that resource depletion at least when it comes to overall output is not evident in this number. To the contrary, these 53,700 kg attest to the relative resilience of Yartsa gunbu. Still, as written since many years, increased or at least stable output can be partially attributed to more areas being ever more intensely searched. These statistics do not allow to factor this in. On the other side, the news release reports that the 53.7 t were a 50 percent year-on-year increase from 2012, which indicates that the harvest in 2012 was a meager 36 t. This low figure does not come as a surprise, since the winter and spring of 2011/2012 was reported to be drier and warmer than usual, too factors that seem to reduce productivity.  All in all, these production amounts are within the range of previously recorded harvests and thus demonstrate that at least in Tibet AR we seem not to witness total resource exhaustion as claimed by some other sources. 

Here a table from my 2011 Caterpillar Fungus (Ophiocordyceps sinensis) Production and Sustainability on the Tibetan Plateau and in the Himalayas. In: Asian Medicine 5 (2009), p. 291–316. (published as 2009)   download

 



For a discussion in detail on the issue of sustainable harvesting in past and present please see my blog-entry from May 4, 2010 [link to the blog: cordyceps-crisis-or-reporting-crisis ?].


Here the news story:

Output of Cordyceps sinensis rises in 2013 

LHASA, Feb.22  The output of Cordyceps sinensis in Tibet in 2013 has reached 53,700 kilograms, a 50 percent year-on-year increase compared with that of 2012, according to the Agricultural Department of Tibet Autonomous Region.

Due to more precipitation and better weather than previous years, this herb enjoyed a facilitative environment and consequently a sharp increase in output.

The Cordyceps sinensis is a swift moth larva with parasitic fungus that grows between 3,000 to 5,000 meters above the sea level. In winter, the fungus hibernates under the soil and in between spring and summer, it grows out of the swift moth and into the shape of herb. That is the reason why it is also called "winterworm summerherb".

Last edited Thu, 03/06/2014 - 16:59

Cordyceps militaris in Arkansas

At a forest walk during the annual North American Mycological Association (NAMA) foray in Harriet, Arkansas, we came across a dozen Cordyceps militaris. Here photos from the specimens we dug up from the soft soil. Some where growing from below leaf debris, some from below moss. We also left several specimens undisturbed in the ground. What is very interesting is that these Cordyceps militaris is growing on Lepidopteran larvae and not pupae as most commonly encountered. The order Lepidoptera includes butterflies and moths. Morphologically they look very much like regular Cordyceps militaris. It will be interesting to find out if DNA analysis shows any difference between Cordyceps militaris growing on pupae or larvae. In general C. militaris is globally distributed, but there are speculations that it is a species-complex rather than an identical species distributed on all continents but Antarctica.

A seven-branched Cordyceps militaris growing out of a Lepidopteran larva photographed right after excavation. It was found by Rachel Giffin and Ken Gribble.

The same Cordyceps militaris specimen well cleaned. Unfortunately, one of the stromata broke off while showing it at the NAMA meeting.

 

Mature fruiting body covered by round knobs, which are the ostioles (openings) of the perithecia, the "organs" in which the spores are produced in asci.


A double header - Most specimen had only one stroma. Out of a dozen only two had two heads, and one with seven "heads".

 The site:

 

The site of some of the Cordyceps militaris specimens in a typical Arkansas oak-hardwood forest.
The arrow shows the location of one Cordyceps militaris. The photo on the left shows the same stroma.

Cross section showing the half-embedded perithecia lined up along the outside. In the upper left side of the exposed stroma one can see perithecia that broke away while slicing the stroma.

 

A single fruiting body of the this insect parasite growing on the larva. It seems that the stroma is attached to the rear end of the larva. 

  Interestingly the stromata of these specimens are growing out of the exoskeleton in several places, but "neck" and rear end seem most common in the few specimens we dug up.

The Cordyceps digging crew: Katie, who found the first specimen and husband Drew Parker and myself Daniel Winkler framed by the Evans brothers Don (on the left) and Larry showing off Jake's Fall pathetic water run. Not pictured Rachel Giffin and Ken Gribble who showed up a moment later.

 

 

 

 

Last edited Wed, 01/08/2014 - 00:14

Bolivian Amazon Cordyceps

Cordyceps-diversity in the Amazon Rain Forest is impressive. Down below Cordyceps and allies we found during the February 2013 MushRoaming Bolivia Tour.
All specimens, unless otherwise noted were found in Madidi National Park or close by around Rurrenabaque. They are all deposited in the National Herbarium of Bolivia, La Paz. Special thanks to Tatiana Sanjuan, Bogota for help with identification.  All photos © Daniel Winkler
 

Cordyceps scarabaeicola

 

 
 

Close-up of the digested scarabaeid beetle.

 

Mature Cordyceps scarabaeicola with two stromata growing out of a scarabaeid beetle. Note how the two stromata are fused half way up.

 

 

  
Close-up of the fertile top of the stroma of Cordyceps scarabeicola. Several fertile cushions, in which perithecioid ascomata are immersed, are growing on top of the stroma.
 The superficial to partially immersed perithecia give the fruiting body of C. scarabeicola a much more irregular appearance, which is more common in Cordyceps than in Ophiocordyceps species.

 

 

 

   Cordyceps sp.  close to C. takaomontana

 

 

Cordyceps takaomontana? in Bolivia

In Mashaquipe we found one location so rich in Cordyceps it blew our minds. We called it "Cordyceps corner". There were about a dozen specimen of at least 5 genera. Most common was this Cordyceps that looks awfully like the East Asian Cordyceps takaomontana first described in Honshu, Japan. However, C. takaomontana was described to grow on coleopteran pupae and the host looks more like a larva.

The number of stromata growing from a leaf surface differed mightily from two to over ten. Interestingly at first, we could not see any insect hosts and were a bit perplexed. Then we realized all of them were leaf-insect sandwiches, the ham was a larva (see below).

 

Fruiting bodies growing out of both side of this "leaf sandwich".

 

 

Cordyceps takaomontana?

The leaves were welded together by mycelium and a challenge to open. Inside we found insects that looked to be in the larval stage.

Coryceps takaomontana stromaClose up of the ascoma showing the bright yellow tops of the perithecia.

Close up of very mature perithecia.

  Ophiocordyceps dipterigena

An already dried out specimen of Ophiocordyceps dipterigena. Seven teleomorphic stromata and one slender anamorphic conidiphore are growing from a fly (order Diptera, hence the species epithet) laying on its back.
The anamorph is also described as Hymenostilbe dipterigena. It was found in Mashaquipe, Madidi NP.

 

Ophiocordyceps sp.

At first we thought, finally we found Ophiocordyceps amazonica. However the host insect does not fit the bill. O. amazonica is a parasite on Orthoptera, meaning locusts, grasshoppers & crickets.

The broken larvae with the typical marking of a cockroach (Blattodea sp.) according to Tatiana Sanjuan.

 

Cordyceps cylindrica

 

Cordyceps that impressed us with its whitish and very geometrically shaped fruiting body. We were blown away seeing it coming forth from the ground out of its own cavity. Well, it turns out it was not its own cavity but apparently this Cordyceps squatted the home of a Mygalomorph trapdoor spider (Araneae) and is successfully digesting the host.


What looks like fine white threads are the spores ejected out of the asci cells.



 

 

Gibellula arachnophila

Gibellula arachnophila

Here a close up of a Gibellula arachnophila, a Cordyceps anamorph we found in the cloud forest near Coroico. Gibellula arachnophila feeds on spiders. A spider is below that white mesh. Gibellula arachnophila produces conidiospores, cloned reproductive cells. Most conidiospores are dispersed already and one can see the conidiphores, the structure on which they grow. Found in Coroico, Yungas, Bolivia.

 
We got more and more excited during exposing the buried cocoon-like structure of this trap door spider. The lid underside is visible to the right ontop of the outer material of the cocoon. Inside the cocoon is a parasitized spider, that might be in a molting process. Very probably this is Cordyceps cylindrica, which is so far reported from East Asia and the United Kingdom.

 
Carefully opening the cocoon two days later revealed a Mygalomorph Trapdoor spider that is being digested by a Cordyceps. Spiders are most susceptible to Cordyceps attacks during molting phases. (Check out more spider Cordyceps I found in Columbia). Since we prevented the cocoon from drying out, this Cordyceps sporulated for us.

  

 

 

 

Akanthomyces novoguineensis 

This could be Akanthomyces novoguineensis, another anamorph that likes to feed on spiders.
Found in Coroico, Yungas, Bolivia.

 

Cordyceps sphecocephala

 

Ascomata of Cordyceps sphecocephala. The spots are the ostiole openings of the perithecia. 

  
Cordyceps sphecocephala is specialized to parasitize wasps, hence its name, "spheco" is derived from attic Greek meaning wasp.

 

 

 

Looking for mushrooms at Wizard Mountain we were accompanied by Fonier and his sons Manuco and Patrick. The boys really got into it!
They found a bunch of Cordyceps, here Patrick looks at a Metacordyceps martialis he found with Larry’s lens, they got to keep in the end.

Note the red-eyed fly resting on the top.

 Metacordyceps martialis

Metacordyceps martialis growing on a larva of a Coleoptera beetle. Metacordyceps martialis clearly has a weakness for the juicy coleopteran larvae that never will get to experience how it feels to transform into a big, well protected rain forest beetle. Coleoptera is largest order in the animal kingdom with more than 350,000 described species. The name is derived from the Greek koleon (κολεον) meaning "sheath" and ptera (πτερα) meaning "wings". Aristotle already called beetles 'koleopteros' (κολεοπτερος) to refer to the front wings modified to protect the membranous hind wings (Coleoptera info from bugguide.net). Already in 2012 we found several of these attractive Metacordyceps martialis at Wizard mountain during Mushroaming Bolivia.

 

 Close-up of the ascoma of Metacordyceps martialis. The fruiting body is still immature and the ascoma with the perithecia is not developed yet. The white top of the stroma is still differentiating. The lower part of the top shows ostioles, future openings through which spores will be released which are produced in the perithecia embedded in the ascoma.

Metacordyceps (Clavacipitaceae) as a genus is still not well established, Kegler et al. (2011) wrote in their paper on Metacordyceps: “Metacordyceps remains one of the most poorly understood”. However, “Anamorph genera Metarhizium and Pochonia were found to be associated only with Metacordyceps and demonstrated to be phylogenetically informative for the clade”.

Ophiocordyceps melolonthae

Ophiocordyceps melolonthae is growing on a coleopteran beetle larva. Found in Coroico, Yungas, Bolivia


Another Ophiocordyceps melolonthae growing also on a coleopteran beetle larva. Found near Mashaquipe, Madidi National Park, Bolivia.

Bright yellow pigmented stroma of Ophiocordyceps melolonthae, the head being clearly distinguished, a clear separation of the ascoma, the fertile tissue with asci cells containing perithecia of the 'head' and lower sterile tissue of the stem.

Same fungus down in the Amazon Rain Forest




Close-up of ascoma and parasitized host, a coleopteran larva.

 

 Ophiocordyceps nutans

The same specimen of Ophiocordyceps nutans showing top and bottom of a Shield or Stink bug (Pentatomidae, Heteroptera)

Another specimen of Ophiocordyceps nutans. Interestingly one of the stroma is a teleomorph, the other the anamorph. In the past the anamorph would have a different species name than the teleomorph, but a new rule says it needs to be the same name, since it is the same organism, just using a different reproductive path. 

The thicker ascoma in the back is the teleomorph. Within its perithecia, asci cells are located in which spores are sexually produced. In the front the anamorph, the asexual stroma of Hymenostilbe nutans.

Ophiocordyceps curculionium

 
Ophiocordyceps curculionium is specialized in attacking weevils. It is closely related to Ophiocordyceps australis, which attacks ants.

Ophiocordyceps australis

Ophiocordyceps evansii, closely related to O. australis. We find these ant Cordyceps most frequently. Also tiny, the bright red ascomata are easily spotted when scanning the forest floor and dead trunks hunting for Cordyceps.

 

Ophiocordyceps%20australis_on_Pachycondila-crassinoda_ant
Ophiocordyceps evansii on Pachycondyla crassinoda ant.

 

Ophiocordyceps australis growing out of an ant with three tall immature stromata and a forth smaller immature branching off below a mature, pin-head shaped ascoma.

Close up of mature and immature stromata.

Ophiocordyceps tiputini

Ophiocordyceps tiputini sp. nov. with yellow ascomata and branched blackish stroma on Megaloptera larva. Tatiana Sanjuan provided the name for this unusual Cordyceps we found in Mashaquipe.

 

 

Unidentified Cordyceps on colopteran larva


Unknown Cordyceps

Another Cordyceps we found in Mashaquipe's Cordyceps corner

 

 

A Cordyceps growing on a Colopteran larva, of which only a part was successfully unearthed.

 

Last edited Sat, 05/21/2016 - 15:53

Colombian Cordyceps Species

Searching the oak (Quercus humboldtii) dominated montane cloud forest in Chicaque near Bogota, Colombia, Tatiana Sanjuan (see below) and I found an array of Cordyceps-species.  We were very lucky since there had been only one good rain that announced the end of the the dry season.

A Cordyceps currently classified with Cordyceps caloceroides, a spider parasite. Its fruiting bodies have pushed open a spider's egg sac. This Cordyceps specimen is only about 5 mm tall.

An other Cordyceps caloceroides.

 

Another Cordyceps caloceroides group Cordyceps growing on an idiopidae spider

 below a close up of the still immature fruiting body.

 

 

A Cordyceps very close to Cordyceps variabilis

Note the sterile apex of the stroma that bears a fertile cushion in which perithecioid ascomata are immersed. The host insect should be a fly larva.

Here a link to a paper by Kathy Hodges, Richard Humber & Chris Wozniack (1998) on Cordyceps variabilis.

 

Isaria anamorphs

Isaria tenuipes (= Paecilomyces tenuipeswhen freshly found. 

 Digging out carefully Isaria tenuipes (=Paecilomyces tenuipesshow the parasitized insects that serve as host. We saw probaly nearly a dozen Isaria during our two day excursion. 

An unidentified Cordyceps anamorph.

 Tatians Sanjuan, a leading expert on neo-tropical Cordyceps, was so kind to take me out in the woods. I had promised to share some of my photography techniques and she delivered Cordyceps objects!
Very interesting was Tatiana's confidence in Cordyceps showing up in the same location year after year.  

 

 

 

Last edited Tue, 04/09/2013 - 10:10

Caterpillar fungus Interview in China Daily Dec.17, 2012

Below I am posting an interview that was published in China Daily, the English language newspaper published by the government of the People's Republic of China. Over four months passed between interview and publication. Speculations regarding the strange title China Daily chose can be found at the end of this blog.

"Threats stem from lack of statistics, over-harvesting"

China Daily  2012-12-17

First Person | Daniel Winkler

China Daily Editor's note: Daniel Winkler, a German ecologist, has researched caterpillar fungus and its economic effects on China for 15 years. A study he conducted in 2005 with Losang Dradul [= Luorong Zhandui] of the China Tibetology Research Center motivated authorities in the Tibet Autonomous Region to introduce a licensing system for fungus harvesting and to keep track of the yearly output. [Not correctly reported: there were already licenses on county level in TAR and data was also collected, but aftersubmission of Luorong's report the first TAR-wide Cordyceps regulation was passed and a conference convened to improve management.]

 

Over-harvesting and poor statistical tracking of caterpillar fungus are major challenges for the sustainable management of the medicinal resource.

Many rural Tibetans rely on this fungus for their incomes, and if anything happens to it, they will face big problems.

Collectors should realize old, low-value caterpillar fungus is better off left in the ground to spread spores for the next year. But at the moment, hundreds of thousands of people are going into the mountains to collect as much as they can. They can’t find everything, but not much is left, and if the situation remains unchecked, the resource will be depleted sooner or later.

Authorities should shorten the harvest season and further limit the number of collectors - for example, only two members should come from each household.

If these restrictions are to work, the key is education. Officials need to work with community leaders to raise awareness to protect the resource so they can benefit for many years to come.

I visited Tibetan communities with collection rights in Yushu prefecture, Qinghai province, with representatives from the China branch of the World Wildlife Fund for Nature. I showed images to villagers to explain the fruiting stages of caterpillar fungus and the different market values.

It only took a few minutes to convince them that the mushroom needs spores to reproduce, contrary to local belief that it just grows by itself every year.

Policymakers should regulate the market to put people off old, low-value caterpillar fungus, which shrinks substantially after it's dried. Then collectors will have the incentive to give up collecting it.

The price rose by 900 percent between 1998 and 2008, when it then dropped 20 percent due to the financial crisis. The rise in price over the past four years is not as high as before, but harvests have fluctuated, as abundant production depends on sufficient rain and snowfall.

There are several research institutes in China devoted to genetic studies and artificial cultivation of caterpillar fungus [but these institutes have NOT succeeded in cultivating caterpillar fungus], which is good news for Tibetan collectors who face serious competition and a price collapse.

However, more resources should be used to find out how to manage the collection of a natural product in a sustainable way, rather than learn how to cultivate artificial ones.

Daniel Winkler was talking to Li Yao and Daqiong. [I have never talked to Daqiong]

 

Daniel Winkler's past publishing notes: 
The sentences in brackets I have added in my blog 
above to correct erroneous statements by Li Yao. 

I never talked to Daqiong, and if he would have been present at the interview in Beijing I am convinced the story would not have had the title they cooked up, since my complain of lack of statistics was aimed at Qinghai Province, the biggest producer of caterpillar fungus as well as the Tibetan areas in Sichuan, Yunnan and Gansu that also lack government efforts to keep track of the most precious product of this region.

Now the title is apparently in juxtaposition to a lengthy article published on the same day in China Daily, to which Daqiong probably contributed substantially. Interestingly the very informative article is full of statistics, never published before. To me the choice of header for my interview in juxtaposition to the TAR caterpillar fungus article seems to try to leave the impression that I was clueless in regard of the statistics kept in TAR. However, in 2008 and 2011 I had published previous years of some of these statistics and my complain regarding lack of statistics was not aimed at TAR, but the other Cordyceps producing areas outside of TAR. However Li Yao did not inform me that China Daily was publishing a second Cordyceps paper on the same day.

Daniel Winkler portrait with Boletus edulis

Ecologist holding a porcini mushroom in 2007.

Daniel Winkler Luorong Zhandui Dawa Dengqen 2005 
Daniel, Luorong Zhangdui, and Dawa from Peking's China Tibet Research Center in Dengqen County, Qamdo Prefecture, TAR, during the Cordyceps field research in 2005. Note the Yartsa gunbu collectors camps on the slopes. (Picture was not in China Daily article)

 

 

 

 

 

Last edited Tue, 04/09/2013 - 10:12

New Research Papers on Himalayan Caterpillar Fungus Collection

Two new papers on Ophiocordyceps sinensis collection in the Himalayas were just recently published, one by C.P. Kuniyal and R.C. Sundriyal on the role of caterpillar fungus in the context of rural livelihood in selected villages in Uttarakhand, the other by U.B. Shrestha & K.S. Bawa on Yartsa gunbu trade harvest and conservation in Nepal's Dolpa region. Both studies offer detailed data on local level shedding light on the situation of the caterpillar fungus industry. Not surprisingly both studies raise the issue of sustainability, an issue looming large after several poor years of harvest. The Dolpa study reports based on over 200 interviews with collectors and traders that 2/3 of the stakeholders are concerned about the sustainability. It offers lots of insight and discusses a whole range of possibilities that could have caused the decline in recorded harvest in the last decade, such as increased collection pressure especially caused by more collectors and increasing value, potential reduction of ghost moth population, climate change etc. Unfortunately there are no answers to what forces are the major factors in harvest reduction, but this a very complex questions and so far there are no studies that would be able to answer this  crucial question. Shresta and Bawa do not offer suggestions how to approach this challenge, whereas the authors of the Uttarakhand study Kuniyal and Sundriyal suggest rotational harvest to diminish negative impact by rotating collection. Though this is no new idea, I have my doubts about feasibility for two reason: firstly, enforcement is very difficult. Keeping collectors off designated areas for 3 out of 4 years seems impossible to me. Secondly, since Yartsa gunbu fruiting is an annual event, host and fungus are dying each year, I am wondering if after three years of fallow, one year of very intense collection might just wipe out the benefits of the fallow. There is still no conclusive empirical research on successful sustainable caterpillar fungus research. In my opinion reducing the continuous collection intensity - be it limiting the collection time to 4 weeks and/or limiting the amount of collectors allowed to enter Yartsa gunbu habitat might be a better match for an organism that infects its hosts each year anew and is distributed in extremely remote areas. However, as Shresta and Bawa point out the build up of functioning local institutions is crucial for creating the capacity of meaningful sustainable management, a process that is in Dolpa underway but far from completed. 

Titles and Abstracts

U.B. Shrestha, K.S. Bawa 2013. Trade, harvest, and conservation of caterpillar fungus (Ophiocordyceps sinensis) in the Himalayas.  Biological Conservation 159 (2013) 514–520.

Abstract: Unsustainable trade in wildlife is regarded as a major driver of biodiversity loss and ecosystem degradation.
Unregulated wildlife trade propels over-exploitation of species, resulting in population declines, and
often in combination with other factors may ultimately extirpate species from their natural habitats.
Concern about the impacts of trade on biodiversity has largely focused on flagship animal species. Here,
we report on the impact of trade on natural populations of the world’s most expensive biological
resource, a unique caterpillar fungus (Ophiocordyceps sinensis). Based on interviews with 203 harvesters
and 28 traders, and focus group discussions in Dolpa, Nepal, we quantify the amount of harvest and trade.
After legalization of trade in Nepal in 2001, trade volume increased persistently, reaching a peak of
2442.4 kg in 2009 and subsequently declining to 1170.8 kg in 2011. The local market price has increased
by up to 2300% over the last 10 years. However, mean annual harvest declined from
260.66 ± 212.21 pieces per person in 2006 to 125.82 ± 96.84 pieces per person in 2010. Our analysis of
harvesters’ perceptions of resource abundance and sustainability shows that virtually all harvesters
(95.1%) believe the availability of the caterpillar fungus in the pastures to be declining, and 67% consider
current harvesting practices to be unsustainable.

Kuniyal, C.P., Sundriyal, R.C. 2013. Conservation salvage of Cordyceps sinensis collection in the Himalayan mountains is neglected.  Ecosystem Services  

Abstract :Traditional agriculture and animal rearing are central in the rural livelihood of Himalayan Mountains.
Economically these activities are inadequate for fulfilling the better livelihood expectation. Therefore rural
people depend on diverse short seasonal activities like collection of medicinal plants, timber trading and
animals poaching etc. for obtaining economic benefits. In past few years, collection of Cordyceps sinensis in
the Himalayan Mountains has been emerged as main short seasonal activity. This practice is more
profitable as compared to the cultivation of key cash crops and some other activities. Because of attractive
economic benefits, nearly 52.08 to 97.98% households of this region are involved in the short seasonal
collection of C. sinensis. Development of C. sinensis in the Himalayan Mountains and economic benefit
earned from its collection is valuable ecosystem service. Uncontrolled collection of C. sinensis will be critical
for its sustainability. Hence rotational pattern for collecting C. sinensis will be useful for its long-term
availability. Because of the shift in short seasonal activities of poor communities, collection of C. sinensis
may be helping in conservation of globally significant medicinal plants (GSMPs), timber yielding trees and
wild animals. However, impacts of this short seasonal activity on the population recovery of GSMPs, timber
trees and wild animals need to be studied at habitat level. Economic benefits earned from short seasonal
collection of C. sinensis and cultivation of key cash crops, suggestion for sustainable collection of C. sinensis,
possible impacts after deviation from this practice and indirect salvage of this short seasonal activity for the
conservation of other resources is discussed in this communication.

 

 

Last edited Thu, 02/21/2013 - 12:33

Cordycepin: Anti-Inflammatory and Anti-Aging Capacity

Recently two interesting research papers on medical propensities of Cordycepin, the most well known of the active ingredients of wild Ophiocordyceps sinensis and also cultivated O.s as well as Cordyceps militaris, were published. The first study presents results of ongoing research at Nottingham University (UK) under the leadership of Dr. Cornelia De Moor. It is entitled: "Inhibition of polyadenylation reduces inflammatory gene induction" and published in RNA. The other paper published in Experimental Gerontology by a mostly Korean team is entitled: "Cordycepin (3′-deoxyadenosine) attenuates age-related oxidative stress and ameliorates antioxidant capacity in rats" and its summary - see below -closes with: "These results suggest that cordycepin is effective for restoring antioxidant status and decreasing lipid peroxidation in aged rats. Aging leads to the gradual loss of pro-oxidant/antioxidant balance and increase oxidative stress. Cordycepin treatment decrease oxidative stress and increase antioxidant status Cordycepin treatment can ameliorate antioxidants during aging and it might attenuate the age-associated disorders".

Now, I do not have the medical back ground to judge the content or the value of these research articles. However, the Nottingham study suggests that Cordycepin has anti-inflammatory capacity that is worth to be investigated further for potential drug development. The Korean study is packed with graphics that leave the impression that negative impact from aging on cellular level has been reduced substantially in rats that have been treated with Cordycepin.

Of course these studies do not address the medical value of natural Yartsa gunbu, the complex of the ghost moth larva taken over by caterpillar fungus - Ophiocordyceps sinensis. However, they clearly indicate that the attention that Yartsa gunbu as a medicinal fungus receives is not made out of thin air.

References with abstracts:

Kondrashov, A., Hedda A. Meijer, Adeline Barthet-Barateig, Hannah N. Parker, Asma Khurshid, Sarah Tessier, Marie Sicard, Alan J. Knox, Linhua Pang, and Cornelia H. De Moor 2012. Inhibition of polyadenylation reduces inflammatory gene induction. RNA 2012. 18: 2236-22502

ABSTRACT
Cordycepin (39 deoxyadenosine) has long been used in the study of in vitro assembled polyadenylation complexes, because it terminates the poly(A) tail and arrests the cleavage complex. It is derived from caterpillar fungi, which are highly prized in Chinese traditional medicine. Here we show that cordycepin specifically inhibits the induction of inflammatory mRNAs by cytokines in human airway smooth muscle cells without affecting the expression of control mRNAs. Cordycepin treatment results in shorter poly(A) tails, and a reduction in the efficiency of mRNA cleavage and transcription termination is observed, indicating that the effects of cordycepin on 39 processing in cells are similar to those described in in vitro reactions. For the CCL2 and CXCL1 mRNAs, the effects of cordycepin are post-transcriptional, with the mRNA disappearing during or immediately after nuclear export. In contrast, although the recruitment of RNA polymerase II to the IL8 promoter is also unaffected, the levels of nascent transcript are reduced, indicating a defect in transcription elongation. We show that a reporter construct with 39 sequences from a histone gene is unaffected by cordycepin, while CXCL1 sequences confer cordycepin sensitivity to the reporter, demonstrating that polyadenylation is indeed required for the effect of cordycepin on gene expression. In addition, treatment with another polyadenyation inhibitor and knockdown of poly(A) polymerase a also specifically reduced the induction of inflammatory mRNAs. These data demonstrate that there are differences in the 39 processing of inflammatory and housekeeping genes and identify polyadenylation as a novel target for anti-inflammatory drugs.

Thiyagarajan Ramesh, Sung-Kwang Yoo, Sung-Won Kim, Seock-Yeon Hwang, Sang-Hyun Sohn, Il-Woung Kim, Si-Kwan Kim 2012. Cordycepin (3′-deoxyadenosine) attenuates age-related oxidative stress and ameliorates antioxidant capacity in rats. Experimental Gerontology Volume 47, Issue 12, December 2012, p. 979–987

ABSTRACT:
Free radical-induced oxidative damage is considered to be the most important consequence of the aging process. The activities and capacities of antioxidant systems of cells decline with increased age, leading to the gradual loss of pro-oxidant/antioxidant balance and resulting in increased oxidative stress. Our investigation was focused on the effects of cordycepin (3'-deoxyadenosine) on lipid peroxidation and antioxidation in aged rats. Age-associated decline in the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), glutathione-S-transferase (GST), reduced glutathione (GSH), vitamin C and vitamin E, and elevated levels of malondialdehyde (MDA) were observed in the liver, kidneys, heart and lungs of aged rats, when compared to young rats. Furthermore, serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), urea, and creatinine were found to be significantly elevated in aged rats compared to young rats. Aged rats receiving cordycepin treatment shows increased activity of SOD, CAT, GPx, GR and GST, and elevated levels of GSH, and vitamins C and E such that the values of most of these parameters did not differ significantly from those found in young rats. In addition, the levels of MDA, AST, ALT, urea and creatinine became reduced upon administration of cordycepin to aged rats. These results suggest that cordycepin is effective for restoring antioxidant status and decreasing lipid peroxidation in aged rats. Aging leads to the gradual loss of pro-oxidant/antioxidant balance and increase oxidative stress. Cordycepin treatment decrease oxidative stress and increase antioxidant status Cordycepin treatment can ameliorate antioxidants during aging and it might attenuate the age-associated disorders.

Last edited Wed, 03/27/2013 - 15:33

Yartsa Gunbu Article in National Geographic Magazine

National Geographic Magazine ran an article solely dedicated to Yartsa Gunbu - Ophiocordyceps sinensis in their August 2012 edition entitled “Tibetan Gold - a medicinal fungus highly prized in China is fueling a boom on the Tibetan Plateau”. The six pages of text were written by Michael Finkel; the seven photos, including four double-page spreads, were taken by Michael Yamashita. Both Michaels have had several NGM assignments before. Also included was a graphic depiction of the life cycle of Ophiocordyceps sinensis and a distribution map.

I probably better disclose at this point that I was contacted in February by the editors to clarify certain points for them. Most of them were pretty straight forward and easy to answer, since most of the factual knowledge in the article is based on the research I have carried out and published over the last 15 years. Actually, I had offered NGM such an article twice, once in 2006 and again in 2009, but I never heard back for both story proposals. Anyway, I was very honored that NGM requested to use my Yartsa Gunbu distribution map as the base for their map. Not surprisingly they have created a much nicer version than I have produced so far. Furthermore, I was in close collaboration with the beautiful design of the life cycle graphic. I have already integrated both graphics into my Cordyceps presentations. Last but not least, I got quoted in the context of Yartsa collection and sustainable management, currently my main concern regarding the Yartsa Gunbu phenomenon.

Overall the article is very informative in regard to the collection and role of Yartsa Gunbu in current Tibetan society as well as its trade in Tibet and beyond, including its use in China. However, the section on its medical uses falls short. Finkel presents the usual spin that there are no proven medical benefits to Cordyceps, probably on the assumption that the readers want to hear that everyone consuming Yartsa gunbu is just crazy and the Tibetans laugh all the way to the bank. It would have been appropriate to dig a bit deeper into the medical Cordyceps research. The U.S. National Library of Medicine’s Pubmed webpage lists for Cordyceps over 700 medical research papers. Each year there are dozens of papers published especially in East Asia. Or what about just a short reference to the Nottingham University study (Wong et al. 2010) that demonstrated how Cordycepin stops cancer cell reproduction, even if it is not proven by clinical trial yet to actually work as a cancer remedy? And just a few months after the article was published National Geographic News published a Cordyceps article by Nicholas Mott entitled:  "Caterpillar Fungus Has Anti-Inflammatory Properties - Tibetan spore could lead to new drugs for cancer, asthma, and diabetes" based on another study (Kondrashov et al. 2012) lead by Cornelia de Moor from Nottingham University. In short, there is a plethora of material available to report on Cordyceps and its medicinal potential in a different tone. Moreover, the author’s attempt to leave a bit of dignity to Yartsa Gunbu users by integrating a story on a Chinese cancer patient that felt much better using Yartsa gunbu failed completely. I have no issue with anecdotal evidence - a friend’s healing experience means more to me than a mere statistic. However, using anecdotal evidence in this article was completely upended by the epilogue that the Chinese cancer patient had died just before the article was published. 

I argued in favor of two editorial inputs which were accepted. This really made me happy. One was the use of the proper Tibetan name for caterpillar fungus “Yartsa gunbu”, a name I have been championing since my first publication on Yartsa gunbu in 2003. I could make the case that it is preferable to stick with the name already published in the 15th Century by Zurkhar Nyamnyi Dorje, a Tibetan doctor who praised Caterpillar Fungus as a superior aphrodisiac (Winkler 2008). Actually when I shared the Tibetan text for translation with my twin brother Jakob, who is a tibetologist, I was hoping to find a source not focusing on the aphrodisiac theme, but that was what Nyamnyi Dorje focused on. The other suggestion NGM accepted was regarding the source region. Originally NGM was going to report that 96.5% of all Yartsa gunbu is produced in China. Although these were my own numbers and it is a correct statement, I pointed out that probably 95% of harvest is from areas designated as autonomous Tibetan areas in China, such as Tibet Autonomous Region (TAR), and the Tibetan Autonomous Prefectures (TAP) located  within Sichuan, Qinghai, Yunnan and Gansu Provinces. Together these autonomous prefectures outside of TAR encompass nearly two thirds of the Tibetan cultural area and the Tibetan population. And nearly all of these areas have Yartsa gunbu resources. The Tibetan areas in Qinghai alone harvest annually more Yartsa gunbu than Tibet AR.

Strangely the author uses Chinese names for all the Tibetan collectors and dealers he interviewed and writes about. The original Tibetan name got mangled beyond recognition for any western reader. How is one to recognize Chinese “Silang” for Tibetan “Sonam”? I thought it could be Tsering - Chinese often turn an “R” into an “L”. Anyway, Sonam has been used in English for centuries already; it is as common a name as Sam in English. For example, there is the 3rd Dalai Lama Sonam Gyatso, the 2nd Panchen Lama Sonam Choklang and the list goes on and on.

Doing field work on Yartsa gunbu since the late 90s I can not help myself and have to analyze the prices reported in the article. Price development of Yartsa gunbu is a very intriguing subject, basically prices went up over 1000% since I am researching Yartsa gunbu, but the 2008 global financial crisis really made a dent in the steady increase and the market has been less bullish. However, two consecutive poor harvests due to dry winters have driven prices up in 2012. As most popular articles on Yartsa gunbu, the NGM article focuses on the highest prices achieved for the precious fungus, however it is mentioned that “Silang and Yangjin”, two collectors the author followed around in Serxu (= Tibetan: Sershul, Chinese: Shiqu), a county in the west of Sichuan’s Garze (= Tibetan: Kandze, Chinese: Ganzi) TAP, received 580 Yuan or $90 for 30 Yartsa pieces, which amounts to 20 Yuan or $3 per piece, contradicting earlier statements. In the intro image a subtitle reads: "Some go for $20 (120 Yuan) apiece". Early on in the text it is stated it commands twice the price of gold in a fancy shop in China, which would indicate a price of $26,500 or 167,000 Yuan per pound and translates to about 210 Yuan per specimen. In the final double page photo of the article it is stated "For 1500 high-quality worms - that's about two pounds - the firm could reap $100,000". Well, we are not just talking high-quality, 750 Yartsa gunbu per pound is the highest quality, they hardly come any bigger than that! And size does matters in the caterpillar fungus trade, I like to speculate there is a connection to its use as an aphrodisiac. Actually, this late May I saw a display of Yartsa gunbu, which is known in China as Chong cao, in the same store claiming it only required 600 pices to weigh in at one meteric pound. I never saw anywhere such an exceptional size class before, just as I never seen the price class before (So far I do not spend time searching for high end stores in Beijing or Shanghai). Zhong Shi Chong Cao Store charged 740,000 Yuan which equals US$ 118,800! Interestingly the store depicted in the article is the same I have been visiting during my MushRoaming Cordyceps Expedition for several years now. I posted basically the same picture of young Chinese clerks bundling caterpillar fungus on my webpages since two years (see also photo below). There is clearly no copyright on photo ideas, and how does the saying go? "Imitation is the sincerest form of flattery".

Towards the end of the article myco-visionary Paul Stamets was quoted as saying "People could be poisoned" from eating wild-crafted Caterpillar Fungus, "for inexperienced it is a form of Russian roulette". I could neither believe that Paul is still claiming this, nor that NGM printed this claim. For starters, Paul's company Fungi Perfecti is selling artificially grown Cordyceps mycelium. Some people would call that a clear conflict of interest. I see no conflict of interest in Paul's absurd statement, as he was being a good businessman, trying to sell his product. However, NGM publishing this seems very odd. As wild caterpillar fungus is a cherished status symbol, which rich and powerful Chinese love to consume. We would hear right away in the media if they were being poisoned by the natural fungus-larva complex. Not a single story surfaced in the last 15 years regarding a Cordyceps poisoning. The only thing that keeps being repeated is a case many, many years ago when some ‘creative’ back country dealer inserted lead wire into caterpillar fungus to add some weight. That scared the whole industry and the big brokers now use x-ray machines to make sure this will not happen again.

Or maybe Paul in his visionary capacity foresaw the news published in The New York Times (October 12, 2012). Gu Kailai, the murderous wife of disgraced Chongqing communist party star Bo Xilai was allegedly ingesting mercury and lead-laced Yartsa gunbu pills, but luckily realized early enough she was being poisoned and thus survived the poison attack! My recommendation: Stay away from pills! It is best to stick to consuming the natural thing if you can afford it and do look for a less dysfunctional family.

Overall, I was very pleased to see National Geographic Magazine finally dedicating a full story to the Yartsa gunbu phenomenon. There is no other cash crop on this globe that is generating such substantial income for an otherwise marginalized local people. For better or worse, there is no other rural economy that has been pushed from subsistence production to a cash economy relying on a traditional resource in such a short period. Of course such changes cause a lot of challenges to any community and it will be interesting to follow the changes and how the communities will fare. Above all, it is crucial to secure sustainable management approaches, something badly neglected so far. Picked for centuries, Yartsa gunbu seems quite resilient, but the current onslaught is unparalleled. To quote the NGM article: “To harvest the worms sustainably, pickers would need to leave some stalks in the soil to mature and infect the next season’s larvae, says ecologist Daniel Winkler”.

 

References:

Finkel, Michael: Tibetan Gold - A medicinal fungus highly prized in Chinais fueling a boom on the Tibetan Plateau (Photographs by Michael Yamashita). National Geographic Magazine. August 2012: 114-129.

Kondrashov, A., Hedda A. Meijer, Adeline Barthet-Barateig, Hannah N. Parker, Asma Khurshid, Sarah Tessier, Marie Sicard, Alan J. Knox, Linhua Pang, and Cornelia H. De Moor 2012. Inhibition of polyadenylation reduces inflammatory gene induction. RNA 2012. 18: 2236-22502

Mott, Nicholas 2012.  Caterpillar Fungus Has Anti-Inflammatory Properties - Tibetan spore could lead to new drugs for cancer, asthma, and diabetes. National Geographic News Nov 28, 2012.

Winkler, D. 2008. The Mushrooming Fungi Market in Tibetexemplified by Cordyceps sinensis and Tricholoma matsutake. Proc. 11th Seminar of International Association for Tibetan Studies, Bonn 2006. Editors Kenneth Bauer, Geoff Childs, Andrew Fischer & Daniel Winkler. In: Journal of the Intern. Assoc. Tibetan Studies / JIATS 4: 1-47. 

Wong, Edward: New Details of How Wife of Chinese Politician Thought She Was Poisoned. New York Times. Oct. 12, 2012

Wong YY, Moon A, Duffin R, Barthet-Barateig A, Meijer HA, Clemens MJ, de Moor CH. 2010: Cordycepin Inhibits Protein Synthesis and Cell Adhesion through Effects on Signal Transduction. Journal of Biological Chemistry 285.4: 2610–2621

 

 

Cordyceps processing Chengdu

Cordyceps being bundled in a Cordyceps store in Chengdu's He Hua Chi Market. Photo Daniel Winkler, May 2010.
Online since June 2010 at the Cordyceps 2010 tour report photo pages.

Source: NGM Cordyceps article webpage. Photo: www.MichaelYamashita.com

Last edited Fri, 12/28/2012 - 15:29

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